Index.dat Suite is a program I use in addition to Window Washer. I'm no computer expert but these two programs run on a daily basis seem to keep my machine running much faster than before I started using them.
http://support.it-mate.co.uk/?mode=Products&p=index.datsuiteLink to faq that addresses your question...
http://mysteryfcm.co.uk/?mode=Articles&date=01-11-2005Index.dat Suite is a free download, but Window Washer costs about thirty dollars.
Edited to add-if you have manually deleted .dat files and your computer is still working well you are probably ok.
If you are running XP and do the Index.dat Suite scan you will probably have as one of the results a line in red reading "pc health". It shouldn't allow you to check that one and delete it, but just make sure you don't check it.